Fig 3.

Heme-stained SDS-PAGE of loosely bound outer surface c-type cytochromes prepared from the wild-type and pilA mutant strains. Shown are wild-type DL1 (Wt), short-isoform (pilA4), long-isoform (pilA5), null (pilA7), and deletion (ΔpilA2) strains. The Omc distribution in samples from the ΔpilA2 and ΔpilA1 pilA deletion strains (48) were found to be comparable (unpublished observation). The protein concentrations in the loosely bound outer membrane preparations were determined with a bicinchoninic acid (BCA) assay, and 3 μg of protein was loaded into each lane.