Table 1.
G. sulfurreducens strains used in this work
| Strain name or genotypea | Descriptionb |
|---|---|
| DL1 (Wt) | Wild type |
| DL100 (isogenic Wt) | Wild-type pilA; Kanr |
| pilA4 (short isoform) | Long isoform start codon mutation; TTG→CCC |
| pilA5 (long isoform) | Short isoform ribosome-binding site mutation |
| pilA6 (prepilin) | Peptidase cleavage site mutation |
| pilA7 (pilA4 and pilA5 double mutant) | Long isoform start codon mutation (TTG→CCC) and short isoform ribosome-binding site mutation |
| ΔpilA2 (in-frame deletion) | In-frame deletion mutation |
a
All strain were generated in this work and were derived from DL1 (9).
b
All strains are Kanr, except DL1.