Table 2.

Plasmids used in this work

Plasmida	Descriptionb	Parent plasmid
pCD341	Source of kanamycin resistance cassette	(43)
pLC1	Expression vector with tacUV5 and lac promoters removed	pRG5 (25)
pLC3	pilA (wild type)	pLC1
pLC8	pilA long isoform start codon mutation (TTG→CCC)	pLC3
pLC13	pilA short isoform ribosome-binding site mutation	pLC3
pLC14	pilA long isoform start codon mutation (TTG→CCC) and short isoform ribosome-binding site mutation	pLC3
pLC17	pilA GSU1497, 1,271 bp of GSU1498	pLC1
pLC18	pilA GSU1497, GSU1498, GSU1499, GSU1500	pLC17
pLC19	pilA GSU1497, GSU1498, GSU1499, GSU1500, GSU1501, GSU1502, GSU1503, GSU1504, GSU1505	pLC18
pLC20	pilA peptidase cleavage site mutation	pLC3

^aAll plasmids were generated in this work except as noted.

^bAll plasmids are Spec^r, except pCD341 (Kan^r).