Table 1.
Plasmids and strains used in this study
| Plasmid or strain | Description or genotype | Reference and/or source |
|---|---|---|
| Plasmids | ||
| pAID3129 | Mini-pLS20 | 4 |
| pAID3169 | Mini-pLS20 alp7A(D212A) | 4 |
| pAID3171 | Mini-pLS20 Δalp7AR | 4 |
| pAID3195 | Mini-pLS20 alp7A-gfp | 4 |
| pAID3204 | pPxylalp7R | This study |
| pAID3219 | pPxylalp7R-gfp | This study |
| pAID3226 | Mini-pLS20 alp7A(D212A) Δalp7R | This study |
| pAID3232 | Mini-pLS20 alp7A(D212A)-gfp Δalp7R | This study |
| pAID3244 | Mini-pLS20 Δalp7R | This study |
| pAID3261 | Mini-pLS20 alp7A-gfp Δalp7R | This study |
| pAID3277 | pPT7His6-alp7R | This study |
| pME7 | pParaalp7R | This study |
| Strains | ||
| B. subtilis | ||
| PY79 | Prototroph, 168 lineage | 53 |
| JP3133 | pAID3129 [mini-pLS20]/PY79 | 4 |
| JP3161 | PY79 thrC::(xylR+ Pxylalp7A-gfperm) | 4 |
| JP3169 | pAID3169/PY79 | 4 |
| JP3171 | pAID3171/PY79 | 4 |
| JP3196 | pAID3195/PY79 | 4 |
| JP3210 | PY79 thrC::(xylR+ Pxylalp7R erm) | This study |
| JP3223 | pAID3219/PY79 | This study |
| JP3233 | pAID3232/JP3210 | This study |
| JP3245 | pAID3261/JP3210 | This study |
| JP3247 | pAID3226/JP3210 | This study |
| JP3248 | pAID3226/PY79 | This study |
| JP3300 | PY79 amyE::(alp7C cat) | This study |
| JP3302 | pAID3244/JP3210 | This study |
| JP3309 | PY79 amyE::(alp7C cat) thrC::(xylR+ Pxylalp7A-gfp erm) | This study |
| JP3311 | pAID3204/JP3309 | This study |
| JP3315 | pAID3204/JP3161 | This study |
| JP3322 | pAID3219/JP3300 | This study |
| E. coli | ||
| JP313 | F−ΔaraBAD-714 [araD139]B/r? Δ(argF-lac)169 λ− e14−flhD5301 Δ(fruK-yeiR)725(fruA25) relA1rpsL150(Strr) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1 | 6 |
| JP3227 | pME7/JP313 | This study |
| JP3277 | pAID3277/BL21(DE3) | This study |