HPLC analysis of succinyl-CoA:benzoate CoA transferase reaction. (A) Time-dependent consumption of 0.2 mM succinyl-CoA (peak 1) and formation of benzoyl-CoA (peak 2) in the presence of 0.5 mM benzoate. Upper panel, after 0.1 min of incubation; lower panel, after 10 min of incubation. (B) Time-dependent consumption of 0.2 mM benzoyl-CoA (peak 2) and formation of succinyl-CoA (peak 1) in the presence of 0.5 mM succinate. Upper panel, after 0.1 min of incubation; lower panel, after 10 min of incubation. (C) Time course of succinyl-CoA:benzoate CoA transferase reaction starting with succinyl-CoA. Solid line, succinyl-CoA; dashed line, benzoyl-CoA.