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SDS-PAGE analysis of succinyl-CoA:benzoate CoA transferase enrichment from the wild type (A) and after heterologous expression of the gene in E. coli (B). (A) Lanes: 1, cell extracts of G. metallireducens; 2, after DEAE chromatography; 3, ceramic hydroxyapatite; 4, MonoQ; 5, Reactive Green agarose. (B) Lanes: 1 and 2, cell extract of E. coli before (lane 1) and after (lane 2) IPTG induction; 3, purified His-tagged protein after Ni-chelating chromatography.
