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Enrichment of succinyl-CoA:benzoate CoA transferasea
| Purification step | Protein (mg) | Sp act (mU mg−1) | Total activity (mU) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|
| Cell extract | 630 | 13 | 7,900 | 100 | 1 |
| DEAE | 118 | 74 | 8,750 | 110 | 6 |
| Hydroxyapatite | 44 | 98 | 4,330 | 55 | 8 |
| MonoQ | 1.2 | 261 | 315 | 4 | 21 |
| Reactive Green | 0.06 | 673 | 37 | 0.5 | 53 |
The starting material was 10 g (wet mass) of wild-type G. metallireducens grown on benzoate. “1 mU” corresponds to the formation of 1 nmol of benzoyl-CoA min−1.
