Table 2.
Substrate preference of heterologously expressed succinyl-CoA:benzoate CoA transferase from G. metallireducens
| Substrate | Activity (%)a |
|
|---|---|---|
| CoA donor: benzoyl-CoA | CoA donor: succinyl-CoA | |
| Succinate | 100 | ND |
| Acetate | <0.1 | <0.1 |
| Malate | <0.1 | ND |
| Fumarate | <0.1 | ND |
| Crotonate | ND | <0.1 |
| Glutarate | ND | <0.1 |
| Benzoate | ND | 100 |
| Cyclohex-1-ene-carboxylate | ND | 126 |
| Cylohexanecarboxylate | ND | <0.1 |
| p-Hydroxybenzoate | ND | 11 |
a
Carboxylic acids (0.125 mM) were tested as CoA acceptors in the presence of benzoyl-CoA or succinyl-CoA as CoA donors. ND, not determined.