Fig 1.

dMβCD as an FC carrier molecule to the H. pylori cell. (A) After H. pylori (10⁶ CFU) was cultured for 24 h with FC beads (FC concentration, 250 μM) in PPLO broth (30 ml) in the presence or absence of 0.2% dMβCD, the H. pylori cells (10⁸ CFU) were recovered to purify membrane lipids, and then the amounts of FC absorbed into the membranes were quantified by the ferrous chloride-sulfuric acid method. The results are indicated as the mean percent FC and standard deviation (SD) (a ratio to the dry weight of total lipid) obtained from three independent experiments. (B) After the same experimental procedures described for panel A were performed, the membrane lipids (200 μg/lane) were analyzed by TLC and detected with a 60% sulfuric acid solution. (C) H. pylori (Hp) or E. coli (Ec) cells (10⁹ CFU) were incubated for 4 h with an FC (100 nmol)-fixed paper disk in the presence or absence of dMβCD (5 mM) in PPLO broth (5 ml). After the cells were recovered and washed to extract the membrane lipids, the FC in the membrane lipids was quantified by the ferrous chloride-sulfuric acid method. The results are indicated as the mean FC and SD obtained from three independent experiments. (D) H. pylori or E. coli cells (10⁹ CFU) were incubated for 4 h in the presence of dMβCD (5 mM) in PPLO broth (5 ml). After the cells were recovered and washed to extract the membrane lipids, the dMβCD in the membrane lipids was quantified by the phenol-sulfuric acid method. The results are indicated as the mean dMβCD and SD obtained from three independent experiments.