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Dependence of protein secretion on HtrA and SAOUHSC_01761. Protein in the three left lanes was isolated from stationary-phase cells incubated in the absence of arylomycin A-C16 (but in the presence of DMSO vehicle) while those in the three right lanes were isolated from cells incubated in the presence of 0.5× their MIC (individually adjusted for each strain) of arylomycin A-C16. Lanes: 1, PAS8001; 2, SCXA81 (ΔhtrA); 3, SCXA108 (ΔSAOUHSC_01761); M, molecular weight marker.
