Table 2.
Oligonucleotides used in this study
| Function and oligonucleotide | Sequence |
|---|---|
| Expression of recombinant proteins | |
| Hb-ST1 | |
| MJS14 | GGAGGAGAATTCCATATGAAACCCTTAATCATCGCCGGT |
| MJS15 | GGAGGAGTCGACTTATTACCCCCCAATATCAGGCGCATCTGG |
| Hb-ST2 | |
| MJS18 | GGAGGAGAATTCCATATGCCACTTAAGCCCCTCATCGTTGCCGGC |
| MJS19 | GAGGAGTCGACTTATTACCCTCCCGTAGCCCTATGGCGTTTAAAC |
| Amplification and sequencing of the GT-42 locus and RT-PCR | |
| NeuAE1 | AAATGGGTGTTAGGCGGTGGTTG |
| NeuAE2 | TAAGCTGGTGTTGGGCGTTAGGG |
| NeuAE3 | AAGTGGCTCTCAGGGCGTGAAAC |
| NeuAE4 | CACCATTAGCGGGCTTTTAGAAGC |
| NeuAE5 | CAGCGTGCATTTGGTTACAGAGC |
| NeuAE6 | ACCAAGTAGAAACCCTAACGCCCAACACC |
| NeuAE7 | ACATCCACCAAGTAGAAACCCTAACGC |
| NeuBE1 | GCCCGGAGCGATGTTAGCAATAG |
| NeuBE2 | GCCCTTAGTGGTGGCTGAAGTGG |
| NeuBE1rc | TTGCTATTGCTAACATCGCTCCG |
| NeuBE3 | AGACTTTGGGACGGGTGGCTAAGGTG |
| NeuCE1 | CGGCTACCCAAATTCAAGCAAGG |
| NeuCE2 | TTTGCCCCGTGCCAAAATGATAG |
| NeuCE3 | TTGTGTGTGGCATGCACCTTTTG |
| NeuCE4 | CACCACTTTCTTGCCCTGCAACG |
| NeuCE5 | TATGACGGATGCTCTCATCTAAAGTCC |
| NeuCE6 | GAGCTTAGCGGGACTTTAGATGAGAG |
| hbs1-a | TAGCCACCTAGTTGCGTGCGATG |
| hbs1-b | TCCAATATGGTCGTTTCTTTCTTGTTGG |
| hbs1-c | CTCTCTGCAATCTTACCCTTGAGCC |
| hbs1-c(rc) | GGCTCAAGGGTAAGATTGCAGAGAG |
| hbs1-d | AATCCGGTGGGAATAAACTATAATCGAGG |
| hbs1-e | TATAGCCTGCATGTCTAGCTCTTGG |
| hbs2-a | AGCGCATAAAGCTGGGCGTATTG |
| hbs2-b | AAGGCTGTAATCTAAATCTTTAATGCTCGG |
| hbs1-fw | CGCAAAGATGGCGGATCGCTTAAC |
| hbs1-rw | TTGTGTGCAGGTCTAAAAAGGGTTTTAGAGTGG |
| promSAB1F | TCTTTACTTGTCCATTGGTTTTGGT |
| promSAB1R | TCTAAACTCCCATTATGGTTGATCC |
| promSAB2F | GATCGAAAATAGAGGCGTTCTTGTA |
| promSAB2R | CTTGGCAATCTCTAAACTCCCATTA |
| RTHBS2rw1 | CCATGTTCAAACCTTTGTAAATGAGATTGTC |
| SSCP/heteroduplex analysis | |
| SSCPNeuCE3b | TTATGCGATCTTTGTGTGTGGCATGCACCTTTTGG |
| SSCPNeuCE5b | ATTTAGACACGCTGTGGCGGATACTCTCATCTAAAGTTC |