Table 3.
CE-ESI-MS in negative-ion mode of LPS-OH from H. bizzozeronii CIII-1 and StorkisTa
| Proposed composition | Observed ion m/z |
Molecular mass (Da) |
Relative abundance (%) |
|||||
|---|---|---|---|---|---|---|---|---|
| [M − 6H]6− | [M − 5H]5− | [M − 4H]4− | [M − 3H]3− | Observed | Calculated | CIII-1 | StorkisT | |
| dHex3·HexNAc2·Hex5·Hep3·PEtn·Kdo·lipid A-OH | 1,199.6 | 3,601.8 | 3,602.47 | 4 | ||||
| dHex3·HexNAc3·Hex5·Hep3·Kdo·lipid A-OH | 1,226.6 | 3,682.8 | 3,683.61 | 2 | ||||
| dHex3·HexNAc3·Hex5·Hep3·PEtn·Kdo·lipid A-OH | 1,267.2 | 3,804.6 | 3,806.66 | 7 | ||||
| dHex3·HexNAc3·Hex6·Hep3·Kdo·lipid A-OH | 1,280.3 | 3,843.9 | 3,845.75 | 4 | ||||
| dHex3·HexNAc3·Hex6·Hep3·PEtn·Kdo·lipid A-OH | 1,321.5 | 3,967.5 | 3,968.80 | 18 | ||||
| dHex3·HexNAc3·Hex7·Hep3·Kdo·lipid A-OH | 1,334.4 | 4,006.2 | 4,007.89 | 9 | ||||
| dHex3·HexNAc3·Hex7·Hep3·PEtn·Kdo·lipid A-OH | 1,375.4 | 4,129.2 | 4,130.94 | 35 | ||||
| Neu5Ac2·HexNAc4 Hex3·dHex·Hep3·Kdo·PEtn2·P·lipid A-OH | 695.6 | 834.7 | 1,043.6 | 1,391.9 | 4,178.8 | 4,178.84 | 93 | |
| dHex4·HexNAc3·Hex7·Hep3·PEtn·Kdo·lipid A-OH | 1,069.1 | 1,424.2 | 4,275.6 | 4,277.08 | 5 | |||
| Neu5Ac2·HexNAc4·Hex3·dHex2·Hep3·Kdo·PEtn2·P·lipid A-OH | 1,080.3 | 4,325.2 | 4,324.98 | 7 | ||||
| Neu5Ac2·dHex3·HexNAc3·Hex7·Hep3·Kdo·lipid A-OH | 1,528.4 | 4,588.2 | 4,590.41 | 3 | ||||
| Neu5Ac2·dHex3·HexNAc3·Hex7·Hep3·PEtn·Kdo·lipid A-OH | 1,177.2 | 1,569.7 | 4,712.5 | 4,713.46 | 13 | |||
a
For the calculation of molecular masses, the following values (in daltons) were used: 1,028.31 for lipid A-OH {based on a β-(1→6)-linked d-glucosaminodisaccharide backbone acylated by (R)-3-hydroxyoctadecanoic acid [18:0(3-OH)] at positions 2 and 2′, carrying a phosphoethanolamine group at position 1), 220.10 for Kdo, 192.17 for Hep, 162.14 for Hex, 146.14 for dHex (deoxyhexose), 203.19 for HexNAc, 291.26 for Neu5Ac, 123.05 for PEtn, and 79.98 for P.