Table 1.
Genotypes and sources of the strains used in the present study
| Strain | Genotype | Source or reference |
|---|---|---|
| HST08 | F−endA1supE44thi-1recA1relA1gyrA96phoA ΔmcrA ϕ80dlacZΔM15 (lacZYA-argF)U169 Δ(mrr-hsdRMS-mcrBC) λ− | TaKaRa Bio Inc. |
| HST08-3 | HST08, supE3 substituting for supE44 | This study |
| HST08-7 | HST08, supE7 substituting for supE44 | This study |
| RFzero-q | HST08, BAC7gent, prfA::zeo | 18 |
| RFzero-q3 | HST08-3, BAC7gent, prfA::zeo | This study |
| RFzero-q7 | HST08-7, BAC7gent, prfA::zeo | This study |
| HST08[ΔsucB] | HST08, sucB::cat | This study |
| RFzero-q[ΔsucB] | RFzero-q, sucB::cat | This study |
| RFzero-q3[ΔsucB] | RFzero-q3, sucB::cat | This study |
| RFzero-iy | BW25113, BAC8, prfA::zeo, piodoTyrRS-MJR1-gent | 19 |
| RFzero-py | RFzero-iy, pPylF (a plasmid expressing a PylRS variant and tRNAPyl) | 19 |