Table 2.

Cm resistance conferred by the supE tRNA variants on DH10B

tRNAc	Base sequenceb	Resistance at Cm concna
		150	200	250	300	350	400	450	500
supE	GAUU—AUUC	+	±	−	−	−	−	−	−
1	GUCU—AAUC	+	+	+	+	+	+	±	−
2, (6)	GCUU—ACUC	+	+	+	+	+	+	+	−
3	GAUU—AAUC	+	+	+	+	+	+	+	−
4	UUCU—AAAG	+	+	+	+	+	+	+	−
5	GGCU—AACC	+	+	+	+	+	+	+	−
7, (8)	CUCU—AAAG	+	+	+	+	+	+	+	−
9, (13, 17)	UACU—AAUA	+	+	+	+	+	+	+	−
10, (12)	GACU—AACC	+	+	+	+	+	+	−	−
11	GCCU—AAUU	+	+	+	+	+	+	+	−
14	ACCU—AACU	+	+	+	+	+	−	−	−
15	ACCU—AAAU	+	+	+	+	±	−	−	−
16	CGCU—AACG	+	+	+	+	+	+	±	−
18	GCCU—AACC	+	+	+	+	+	+	±	−

^aCm concentration (mg/liter) in the growth medium. +, resistance; ±, weak resistance; −, susceptibility.

^bThe base sequence from positions 29 to 40, with the invariant anticodon sequence omitted. Bold letters represent the nucleotides that differ from those of the supE tRNA at the corresponding positions.

^cThe number(s) in parentheses shows the second (and third) clone(s) with the same sequence.