Table 2.
tRNAc | Base sequenceb | Resistance at Cm concna |
|||||||
---|---|---|---|---|---|---|---|---|---|
150 | 200 | 250 | 300 | 350 | 400 | 450 | 500 | ||
supE | GAUU—AUUC | + | ± | − | − | − | − | − | − |
1 | GUCU—AAUC | + | + | + | + | + | + | ± | − |
2, (6) | GCUU—ACUC | + | + | + | + | + | + | + | − |
3 | GAUU—AAUC | + | + | + | + | + | + | + | − |
4 | UUCU—AAAG | + | + | + | + | + | + | + | − |
5 | GGCU—AACC | + | + | + | + | + | + | + | − |
7, (8) | CUCU—AAAG | + | + | + | + | + | + | + | − |
9, (13, 17) | UACU—AAUA | + | + | + | + | + | + | + | − |
10, (12) | GACU—AACC | + | + | + | + | + | + | − | − |
11 | GCCU—AAUU | + | + | + | + | + | + | + | − |
14 | ACCU—AACU | + | + | + | + | + | − | − | − |
15 | ACCU—AAAU | + | + | + | + | ± | − | − | − |
16 | CGCU—AACG | + | + | + | + | + | + | ± | − |
18 | GCCU—AACC | + | + | + | + | + | + | ± | − |
Cm concentration (mg/liter) in the growth medium. +, resistance; ±, weak resistance; −, susceptibility.
The base sequence from positions 29 to 40, with the invariant anticodon sequence omitted. Bold letters represent the nucleotides that differ from those of the supE tRNA at the corresponding positions.
The number(s) in parentheses shows the second (and third) clone(s) with the same sequence.