Table 3.

Growth of RFzero-q strains transformed with the cat genes on Cm-containing plates^a

Strain	cat gene/plasmid	Growth at CM concnb
		100	200	300	400	500
HST08	WT/pACYC184-kan	+	+	+	+	±
RFzero-q	WT/pACYC184-kan	+	+	+	+	±
RFzero-q	cat(13amb)/pCAT(13amb)	+	+	±	−	−
RFzero-q3	cat(13amb)/pCAT(13amb)	+	+	+	+	−
RFzero-q7	cat(13amb)/pCAT(13amb)	+	+	±	−	−

^aHST08 and its derivatives generally exhibit lower levels of Cm resistance than DH10B, when transformed with cat.

^bCm concentration (μg/ml) in the LB medium.+, resistance; ±, weak resistance; −, susceptibility.