Fig 4.
Role of catalytic residues C246 and H184 in SrtC2-mediated FimA polymerization. Membrane (A) and cell wall (B) fractions were collected from wild-type MG-1 and its isogenic derivative strains carrying a deletion of srtC2 or the derivative-expressing SrtC2 mutants. Equivalent protein samples were separated on a 4 to 12% Tris-glycine gradient gel and detected by immunoblotting with α-SrtC2 and α-SrtA (A) and α-FimA (B). The positions of FimA monomers (FimAM), FimA high-molecular-weight polymers (FimAP), and molecular mass markers are indicated.