Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| A. oris strains | ||
| MG1 | Type strain, expressing type 1 and 2 fimbriae | 20 |
| AR1 | ΔsrtC2; an isogenic derivative of MG1 | 20 |
| AR2 | AR1 containing pSrtC2 | 22 |
| WU10 | AR1 containing pSrtC2VV | This study |
| MR1 | AR1 containing pSrtC2TT | This study |
| MR2 | AR1 containing pSrtC2-L45P | This study |
| MR2 | AR1 containing pSrtC2Δsp | This study |
| WU11 | AR1 containing pSrtC2Δ3 | This study |
| WU12 | AR1 containing pSrtC2Δ5 | This study |
| WU13 | AR1 containing pSrtC2Δ59 | This study |
| WU14 | AR1 containing pSrtC2Δ110 | This study |
| WU15 | AR1 containing pSrtC2-H184A | This study |
| WU16 | AR1 containing pSrtC2-C246A | This study |
| Plasmids | ||
| pSrtC2 | pJRD215 derivative expressing wild-type SrtC2 from MG1 | 20 |
| pH 6-SrtA | pMCSG7 derivative expressing SrtA (residues 52 to 253) for antibody production | This study |
| pUC-SrtC2 | The srtC2 fragment from pSrtC2, cut by SalI/HindIII and cloned into pUC19 | This study |
| pSrtC2ΔVV | pJRD215 derivative expressing SrtC2 that carries mutations of SALVTS to VVVVVV at positions 36 to 41 | This study |
| pSrtC2ΔTT | pJRD215 derivative expressing SrtC2 that carries mutations of SIMALVGM to TTTTTTTT at positions 41 to 48 | This study |
| pSrtC2ΔSP | pJRD215 derivative expressing SrtC2 lacking the first 44 residues | This study |
| pSrtC2ΔTM | pJRD215 derivative expressing SrtC2 lacking the residues from 285–302 (transmembrane region) | This study |
| pSrtC2Δ5 | pJRD215 derivative expressing SrtC2 lacking the last 5 residues | This study |
| pSrtC2Δ59 | pJRD215 derivative expressing SrtC2 lacking the last 59 residues | This study |
| pSrtC2Δ110 | pJRD215 derivative expressing SrtC2 lacking the last 59 residues | This study |
| pSrtC2-L45P | pJRD215 derivative expressing SrtC2 with L45P mutation | This study |
| pSrtC2-E104A | pJRD215 derivative expressing SrtC2 with E104A mutation | This study |
| pSrtC2-N106A | pJRD215 derivative expressing SrtC2 with N106A mutation | This study |
| pSrtC2- H184A | pJRD215 derivative expressing SrtC2 with H184A mutation | This study |
| pSrtC2- C246A | pJRD215 derivative expressing SrtC2 with C246A mutation | This study |
| pSrtC1 | pJRD215 derivative expressing wild-type SrtC1 from MG1 | 32 |
| pSrtC1-D129A | pJRD215 derivative expressing SrtC1 with D129A mutation | This study |
| pSrtC1-W131A | pJRD215 derivative expressing SrtC1 with W131A mutation | This study |