Table 2.
Primers used in this study
| Primer | Sequencea |
|---|---|
| LIC-SrtA-5 | TACTTCCAATCCAATGCAATTGACGCCAACGCCAGC |
| LIC-SrtA-3 | TTATCCACTTCCAATGTTAGGTCGTTGAGCACGGACT |
| SrtC2ΔVV-F | GTCGTCATCATGGCGCTGGTGGGCATG |
| SrtC2ΔVV-R | GACCACGACGACGACCGACAGGCGCAGGCGCGGGG |
| SrtC2Δsp-F | CTGGTCACCTCCATCATGGCGCTG |
| SrtC2Δsp-R | CATGGGGCCTCGTACGCTTCCA |
| SrtC2ΔTT-F | ACCACGACCACGACCGGGCTGCTGACCTATCCGACG |
| SrtC2ΔTT-R | GGTGGTGGTGGTGACCAGGGCGGAGACCGA |
| SrtC2-L45P-F | CCGGTGGGCATGGGGGTGGTG |
| SrtC2-L45P-R | CGCCATGATGGAGGTGGAGAC |
| SrtC2ΔTM-F | CGCTCGGGCTACGCGGCGGCGA |
| SrtC2ΔTM-R | GGGGAAGTGGGGAACGTCGGG |
| SrtC2Δ5-F (SalI) | GGCGGTCAGCCTACGTCCTGGTTGAGACC |
| SrtC2Δ5-R (HindIII) | GGCGAAGCTTCTACCCACTGGTCCCCGCGCTCGG |
| SrtC2Δ59-R (HindIII) | GGCGAAGCTTCTAGTCGGTGAACCACCGTTGGGGGCT |
| SrtC2Δ110-R (HindIII) | GGCGAAGCTTCTACCACAGGTACAGCCCGACGAC |
| SrtC2-E104A-F | GCGGCCAACAACCACGTCCCCACC |
| SrtC2-E104A-R | CAGGACCGCCCCGGCCGAGAG |
| SrtC2-N106A-F | GCCAACCACGTCCCCACCGGTGCCG |
| SrtC2-N106A-F | GGCCTCCAGGACCGCCCCGGCCG |
| SrtC2-H184A-F | GCC CGCGGCCTGGCCGAGGCC |
| SrtC2-H184A-R | CCCCGTGATGACCGAGCGGGTC |
| SrtC2-C246A-F | GCCACGCCCCTGGGCATCAACACC |
| SrtC2-C246A-R | GGTCACCAGGGTGAGCAGGTC |
| SrtC1-D129A-F | GAGAGCGCGCCGATCCTCGCCCCCTGGCTCGAGTCGCAG |
| SrtC1-D129A-F | CAGGTTGTTGTTGTAGGTCTCGGCGGAGGCGCGCTCCTT |
| SrtC1-W131A-F | GCGCCGATCCTCGACCCCGCCCTCGAGTCGCAGCGCCCC |
| SrtC1-W131A-R | GCTCTCCAGGTTGTTGTTGTAGGTCTCGGCGGAGGCGCG |
a
Restriction sites in the primers are underlined.