Fig 4.

Overexpression of HA-ubiquitin partially restores virus replication. (A) Effect of HA-ubiquitin overexpression on HEV replication in the context of MG132 treatment. In six-well plates, cotransfection of capped RNA transcripts of the pSK-HEV2RLuc clone and pRK-HA-Ub/pTrix-neo was carried out. MG132 treatment started 1 day posttransfection. A luciferase assay was performed on the fifth day posttransfection. Mean relative light unit (RLU) values from three independent experiments are plotted. Statistical analysis was performed using analysis of variance followed by contrast procedure, and significance was set at a P level of <0.05 (indicated with an asterisk). Data analysis was performed using JMP9 software. (B) Representative results from an immunoblot assay performed with the anti-HA tag monoclonal antibody of the experiment shown in panel A.