Fig 1.

Strategy for inhibiting human cytomegalovirus replication with siRNAs. (A) Schematic diagram representing the organization of the HCMV genome and the relative position of ORFs encoding the UL123/UL122 gene products, IE1 and IE2. Gray bars, exonic structure (exons 1 through 5) of the three major transcripts generated at IE times of infection. The X3 siRNA was designed to target exon 3, which is shared by these transcripts. UL, unique long, and US, unique short (regions of the HCMV genome); MIEP, major IE promoter. (B) Temporal targeting of siRNAs characterized in this study along with most anti-HCMV drugs. UL54, siRNA against UL54-57 transcripts.