Fig 10.

Inhibition of HCMV replication by therapeutic treatment with X3 or UL54 siRNAs. U373MG cells were therapeutically transfected with X3 (A and B), UL54A (C and D) or UL54B (D; also see Fig. S6 in the supplemental material), or CON siRNA at 24 hpi with HCMV at an MOI of 0.3. (A and C) The levels of viral IE, E, and E/L protein expression were assessed by immunoblot analysis for IE1/IE2, pp65, and gB55, respectively. (B and D) Culture supernatants and clarified cell lysates were combined and assayed for infectious virus production by plaque assay.