Fig 2.

Inhibition of IE gene expression by siX3. HEL fibroblasts were untransfected or transfected with X3 or CON siRNAs. Twenty-four hours after transfection, the cells were infected with HCMV AD169 at an MOI of 0.1 (A) or an MOI of 1.0 (B). At 24, 48, 72, and 96 hpi, total RNAs were extracted and analyzed for IE1- and IE2-specific transcripts by Northern blot hybridization with biotin-labeled probes to IE1 (exon 4) or IE2 (exon 5). Arrows mark the 1.7- and 2.25-kb IE2 transcripts. GAPDH is shown as a loading control. The boxed region labeled with an asterisk shows a longer exposure of that region of the membrane.