Fig 3.
Inhibition of cell-to-cell spread of HCMV by siX3 as measured by plaque reduction assay. HEL fibroblasts were transfected with X3 or CON siRNA at a dose of 20, 40, 100, 200, 300, or 400 pmol per 3.6 × 106 cells. The cells were seeded at 100% confluence and infected with ∼40 PFU of virus. After adsorption, cells were overlaid with agarose. After 7 days of incubation, viral titers were determined by staining and counting plaques. Histograms show the averages of three independent experiments, and the error bars denote the standard deviations. *, P < 0.022; **, P < 0.002; ***, P < 0.001.