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. 2012 May;86(10):5660–5673. doi: 10.1128/JVI.06338-11

Fig 4.

Fig 4

siX3 pretreatment compromises HCMV replication. HEL fibroblasts were untransfected or transfected with X3 or CON siRNAs 24 h prior to infection with HCMV at an MOI of 0.1 (A and B) or 1.0 (C and D). (A and C) The levels of viral IE, E, and E/L protein expression were assessed by immunoblot analysis for IE1/IE2, pp65, and gB55, respectively. (B and D) Culture supernatants were assayed for infectious virus production by plaque assay. (E and F) U373MG cells were untransfected or transfected with X3 or CON siRNAs 24 h prior to infection with HCMV at an MOI of 0.3. (E) The levels of viral IE, E, and E/L protein expression were assessed by immunoblot analysis for IE1/IE2, pp65, and gB55, respectively. (F) Culture supernatants and clarified cell lysates were combined and assayed for infectious virus by plaque assay. Boxed areas in panels A and C that are labeled with asterisks show longer exposures of those regions of the immunoblotted membranes.