Fig 6.

Inhibition of viral DNA replication by siX3 and siUL54 pretreatment. HEL fibroblast cells were untransfected or transfected with UL54A, UL54B, X3, or CON siRNAs 24 h prior to infection with HCMV at an MOI of 0.1 (A) or 1.0 (B). The cell pellets were harvested at the indicated times p.i. Total DNA was extracted from infected cells, and equivalent concentrations of DNA were slotted in each well of the slot blot as a measure of viral DNA replication by hybridization with a biotin-labeled probe to UL70.