Comparisons of EV71 clinical isolates. (A) Viral titers of EV71 clinical isolates in RD cells and L-SCARB2 cells. Viral titers of clinical isolates of the EV71 strains 962-Yamagata (962), 786-Yamagata (786), 1002-Yamagata (1002), 983-Yamagata (983), 452-Yamagata (452), and 75-Yamagata (75) were determined with RD cells and L-SCARB2 cells. The data are shown as mean viral titers with SD (n = 3). (B) Comparison of amino acid sequences of the P1 region. Amino acid sequences of EV71 strains 962-Yamagata (subgenogroup B4), 1002-Yamagata (C2), and 452-Yamagata (C4) (upper lines) were compared with those of strains 786-Yamagata (B4), 983-Yamagata (C2), and 75-Yamagata (C4) (lower lines). Only amino acids divergent among the 6 isolates are shown.