Fig 3.

Both the extrinsic and the intrinsic caspase pathways are involved in apoptosis in MVA-infected ALDC. (A and B) ALDC (1 × 10⁶) were infected with rMVA-Ag85A or UV-inactivated rMVA-Ag85A (MOI = 3) at 4°C for 60 min to allow virus attachment; excess virus was removed by extensive washing, and cells were cultured at 37°C. Aliquots were collected at various time points and cell lysates tested for caspase 8 (A) and caspase 9 (B) activity by ELISA. (C to E) ALDC (1 × 10⁶) were infected with rMVA-Ag85A (MOI = 3) as described above in the presence of caspase 8 inhibitor (C), caspase 9 inhibitor (D), or both (E). Graphs represent means of results from cells from 5 different animals analyzed in duplicate. Error bars indicate standard deviations.