Fig 9.

MVA-infected ALDC promote chemotaxis of noninfected DC and T cells. Tissue culture supernatants from mock-infected or MVA-infected ALDC (4 or 24 h of infection) were plated in triplicate onto the lower chamber of a trans-well assay plate. FACS-sorted ALDC (1 × 10⁵) (n = 6) or T cells (n = 5) were then plated onto the upper chamber of the trans-well assay plate and incubated for 4 h at 37°C. Migration to the lower chamber was measured by flow cytometry. (A) Dendritic cell migration. (B) T cell migration. (C) Steady-state staining of ALDC showing expression of SIRPα and CD21. (D) Dot plot showing the phenotype of migrated DC. (E) Isotype controls. Dot plots are representative of 6 independent experiments performed in triplicate.