Fig 2.

Cross-protection ability conferred by immunization with CApH1N1. Two doses of 10⁵ PFU of CApH1N1 or PBS were inoculated intranasally into mice (n = 6 per group) 2 weeks apart. Two weeks after the second inoculation, mice were challenged with five times the mouse 50% lethal dose (5 MLD₅₀) of A/Brisbane/59/2007 (H1N1) and A/Aquatic bird/Korea/W81/2005 (MA81) (H5N2). Daily weight changes (left) and survival rates (right) of mice challenged with A/Brisbane/59/2007 (H1N1) (A) or A/Aquatic bird/Korea/W81/2005 (MA81) (H5N2) (B) were monitored for 2 weeks after the challenge. Data represent the means of the results determined for each cohort (n = 6), and error bars indicate ±SD.