Fig 6.
REST inhibits E1A-mediated cell proliferation and anchorage-independent growth. (A) Inhibition of cell proliferation by REST. 12-1 cells were transfected with the empty vector or a plasmid expressing N-terminally Myc-tagged REST (Myc-REST). Following transfection, 2 × 105 cells were seeded in triplicate and counted at the indicated times of culture. (B) Inhibition of anchorage-independent colony formation by REST. Two thousand cells from the experiment described in panel A were subjected to a soft-agar assay in quadruplicate. Anchorage-independent cell colonies were counted after culture for 4 weeks. Representative pictures of the cell colonies are shown at the bottom. (C) Western blot analysis of REST. Total lysates of transfected 12-1 cells as described in panel A were analyzed by Western blotting using antibodies against REST and β-actin separately.