Table 2.
Neutralization IC50s of autologous serum IgG, autologous CD4bs MAbs, and CD4 surrogate protein CD4-Ig for donor 45 Env variants
| env isolation time, source, and env clone or parameter | Neutralization IC50 (μg/ml) or breadth |
|||||||
|---|---|---|---|---|---|---|---|---|
| Autologous serum IgG |
Autologous CD4bs MAbs |
CD4 surrogate CD4-Ig | ||||||
| 2/8/2001 | 7/14/2006 | 6/2/2009 | VRC01 | VRC03 | VRC06 | VRC06b | ||
| 2001, provirus | ||||||||
| 45_01dG5 | 3.8 | 10.9 | 9.5 | 0.020 | 0.010 | 0.019 | 0.010 | 0.256 |
| 45_01dH1 | 58.6 | 61.4 | 77.0 | 3.6 | 2.2 | 6.9 | 3.9 | 0.870 |
| 45_01dH5 | 11.6 | 18.4 | 16.8 | 0.049 | 0.028 | 0.149 | 0.207 | >50 |
| Breadtha (n = 3) | 3 (100) | 3 (100) | 3 (100) | 3 (100) | 3 (100) | 3 (100) | 3 (100) | 2 (67) |
| 2001, plasma | ||||||||
| 45_01A8 | 310 | 47.6 | 13.0 | >50 | >50 | >50 | >50 | 1.3 |
| 45_01A10 | >1,000 | 26.2 | 12.3 | >50 | >50 | 0.248 | 0.925 | 3.6 |
| 45_01A14 | 121 | 39.8 | 21.0 | >50 | 0.453 | >50 | 1.5 | 1.7 |
| 45_01A21 | >1,000 | 22.9 | 65.6 | >50 | >50 | >50 | >50 | 4.4 |
| 45_01B14 | >1,000 | 14.1 | 68.2 | >50 | >50 | >50 | >50 | 5.3 |
| 45_01E8 | 11.0 | 0.823 | 15.9 | >50 | >50 | 9.4 | >50 | 0.265 |
| 45_01E11 | 201 | 129 | 146 | >50 | >50 | 2.3 | 1.8 | 0.698 |
| 45_01E17 | 109 | 33.0 | 83.2 | >50 | >50 | 3.6 | 3.3 | 1.8 |
| 45_01F4 | 47.3 | 54.2 | 83.2 | >50 | >50 | 7.8 | 10.7 | 0.533 |
| Breadth (n = 9) | 6 (67) | 9 (100) | 9 (100) | 0 (0) | 1 (11) | 5 (56) | 5 (56) | 9 (100) |
| 2006, plasma | ||||||||
| 45_06A1 | 689 | >1,000 | 17.2 | >50 | >50 | >50 | >50 | 3.4 |
| 45_06A2 | >1,000 | >1,000 | 9.0 | >50 | >50 | >50 | >50 | 2.8 |
| 45_06A3 | >1,000 | >1,000 | 9.6 | 17.5 | >50 | >50 | >50 | 2.5 |
| 45_06A4 | 542 | 109 | 225 | >50 | >50 | >50 | >50 | 6.0 |
| 45_06A8 | 254 | >1,000 | 22.4 | >50 | >50 | >50 | >50 | 0.614 |
| 45_06A10 | 640 | >1,000 | 14.5 | >50 | >50 | >50 | >50 | 5.3 |
| 45_06C7 | >1,000 | >1,000 | >1,000 | 31.2 | >50 | >50 | >50 | 3.3 |
| 45_06C8 | >1,000 | 165 | 205 | >50 | >50 | >50 | >50 | 1.4 |
| Breadth (n = 8) | 4 (50) | 2 (25) | 7 (88) | 2 (25) | 0 (0) | 0 (0) | 0 (0) | 8 (100) |
| 2009, plasma | ||||||||
| 45_09A5 | >1,000 | 462 | 879 | >50 | >50 | >50 | >50 | >50 |
| 45_09A6 | >1,000 | 447 | 905 | >50 | >50 | 5.5 | 38.4 | >50 |
| 45_09A10 | >1,000 | >1,000 | >1,000 | >50 | >50 | >50 | >50 | 5.2 |
| 45_09B3 | >1,000 | >1,000 | 456 | >50 | 35.7 | 3.2 | >50 | 4.0 |
| 45_09C5 | >1,000 | >1,000 | 564 | >50 | >50 | >50 | >50 | 2.7 |
| 45_09C8 | >1,000 | >1,000 | 743 | >50 | >50 | >50 | >50 | >50 |
| 45_09C20 | 211 | 178 | 209 | >50 | >50 | >50 | >50 | 0.809 |
| 45_09C26 | >1,000 | >1,000 | >1,000 | >50 | >50 | >50 | >50 | 5.5 |
| 45_09TA1 | >1,000 | 785 | 560 | >50 | >50 | 2.3 | >50 | 9.1 |
| Breadth (n = 90) | 1 (11) | 4 (44) | 7 (78) | 0 (0) | 1 (11) | 3 (33) | 1 (11) | 6 (67) |
a
Breadth is shown as the number (percentage) of Env variants sensitive to the corresponding serum IgG, MAb, or CD4-Ig.