Table 3.
Neutralization IC50s of heterologous CD4bs MAbs and CD4 surrogate protein CD4-Ig for Env variants from five donors
| Donor, yr, and env clone or parameter | Neutralization IC50 or breadth |
|||||
|---|---|---|---|---|---|---|
| VRC01 | VRC03 | VRC06 | VRC06b | b12 | CD4-Ig | |
| 1 | ||||||
| 1995 | ||||||
| 1_95A21 | >50 | >50 | >50 | >50 | >50 | >50 |
| 1_95C1 | >50 | >50 | >50 | >50 | >50 | 13.6 |
| 1_95C5 | 0.017 | 0.020 | 3.3 | 0.016 | 0.023 | >50 |
| 1_95C10 | >50 | >50 | >50 | >50 | 0.214 | 3.7 |
| 1_95TC1 | >50 | >50 | >50 | >50 | >50 | >50 |
| 1_95TC6 | >50 | >50 | >50 | >50 | >50 | >50 |
| 1_95TC13 | >50 | >50 | >50 | >50 | 0.773 | 1.4 |
| 1_95TC14 | 14.6 | 5.0 | 26.2 | 7.1 | >50 | 28.0 |
| 2001 | ||||||
| 1_01A10 | >50 | >50 | >50 | >50 | >50 | 38.6 |
| 1_01A19 | >50 | >50 | >50 | >50 | >50 | 1.9 |
| 1_01A20 | 11.5 | 12.0 | >50 | >50 | >50 | 2.6 |
| 1_01B3 | >50 | >50 | >50 | >50 | 0.920 | 1.4 |
| 1_01B5 | 0.041 | 0.111 | >50 | 0.128 | 0.032 | 2.6 |
| 1_01TB5 | >50 | >50 | >50 | >50 | >50 | 3.2 |
| 2006 | ||||||
| 1_06B1 | >50 | >50 | >50 | >50 | 6.1 | 0.995 |
| 1_06B3 | >50 | 0.841 | 13.1 | 2.5 | >50 | 19.3 |
| 1_06B7 | >50 | >50 | >50 | >50 | >50 | 22.8 |
| 1_06B9 | >50 | >50 | >50 | >50 | >50 | 0.218 |
| 1_06C3 | >50 | >50 | >50 | >50 | 0.289 | 7.8 |
| 1_06C5 | >50 | >50 | >50 | >50 | 50.0 | 2.8 |
| 1_06D29 | >50 | >50 | >50 | >50 | 6.3 | 1.7 |
| Breadtha (n = 21) | 4 (19) | 5 (24) | 3 (16) | 4 (19) | 9 (47) | 17 (81) |
| N26, 2007 | ||||||
| N26_07A10 | >50 | >50 | >50 | >50 | >50 | 9.5 |
| N26_07A14 | >50 | >50 | >50 | >50 | >50 | 0.317 |
| N26_07A16 | >50 | >50 | >50 | >50 | >50 | 2.8 |
| N26_07A21 | 1.6 | 1.7 | >50 | 10.9 | 0.640 | 2.9 |
| N26_07A22 | >50 | >50 | >50 | >50 | >50 | >50 |
| N26_07A38 | 0.591 | >50 | >50 | >50 | 1.1 | >50 |
| N26_07B9 | >50 | >50 | >50 | >50 | 7.2 | 3.9 |
| N26_07B10 | >50 | >50 | >50 | >50 | 7.9 | >50 |
| N26_07B18 | 19.0 | >50 | >50 | >50 | >50 | 0.755 |
| N26_07B22 | >50 | >50 | >50 | >50 | >50 | 2.9 |
| N26_07B36 | >50 | >50 | >50 | >50 | >50 | 3.1 |
| N26_07B41 | >50 | >50 | >50 | >50 | >50 | 12.9 |
| N26_07TC39 | 19.3 | >50 | >50 | >50 | >50 | 44.4 |
| N26_07TC40 | 0.089 | >50 | >50 | >50 | 10.4 | >50 |
| Breadth (n = 14) | 5 (36) | 1 (7) | 0 (0) | 1 (7) | 5 (36) | 10 (71) |
| B7B5, 1988 | ||||||
| B7B5_88A1 | 17.0 | 38.7 | >50 | >50 | >50 | 1.5 |
| B7B5_88A2 | 2.3 | 6.9 | >50 | 15.5 | 0.452 | 25.3 |
| B7B5_88A3 | 3.1 | 12.6 | >50 | >50 | 0.426 | 13.7 |
| B7B5_88A4 | >50 | >50 | >50 | >50 | 0.731 | 0.119 |
| B7B5_88A5 | >50 | 20.0 | >50 | >50 | >50 | 6.9 |
| B7B5_88A6 | 3.5 | 7.5 | >50 | 19.1 | 0.141 | 0.970 |
| B7B5_88A7 | >50 | >50 | >50 | >50 | 36.1 | 9.6 |
| B7B5_88A10 | 25.9 | >50 | >50 | >50 | >50 | 7.3 |
| B7B5_88TB1 | >50 | >50 | >50 | >50 | >50 | 11.4 |
| B7B5_88TB2 | 9.6 | 2.2 | 5.3 | 1.6 | >50 | 8.8 |
| B7B5_88TB3 | 11.4 | 2.7 | 8.7 | 1.6 | 4.4 | 6.9 |
| B7B5_88TB6 | >50 | >50 | >50 | >50 | >50 | 2.4 |
| Breadth (n = 12) | 7 (58) | 7 (58) | 2 (17) | 4 (33) | 6 (50) | 12 (100) |
| 18, 1999 | ||||||
| 18_99A27 | 0.661 | >50 | >50 | >50 | >50 | >50 |
| 18_99A34 | 3.5 | 1.9 | >50 | >50 | >50 | >50 |
| 18_99A38 | >50 | >50 | >50 | >50 | >50 | >50 |
| 18_99B1 | 4.8 | >50 | >50 | >50 | 9.6 | 13.5 |
| 18_99B15 | 1.9 | 1.5 | >50 | >50 | >50 | >50 |
| 18_99B19 | 0.855 | >50 | >50 | >50 | >50 | 43.1 |
| 18_99B27 | >50 | >50 | >50 | >50 | >50 | 10.2 |
| 18_99B39 | >50 | >50 | >50 | >50 | >50 | 28.8 |
| 18_99B46 | 1.6 | >50 | >50 | >50 | >50 | >50 |
| 18_99B48 | >50 | >50 | >50 | >50 | >50 | 3.1 |
| 18_99B51 | 1.2 | >50 | >50 | >50 | >50 | >50 |
| 18_99TA3 | >50 | >50 | >50 | >50 | >50 | 7.2 |
| 18_99TB13 | 1.1 | >50 | >50 | >50 | >50 | >50 |
| Breadth (n = 13) | 8 (62) | 2 (15) | 0 (0) | 0 (0) | 1 (8) | 6 (46) |
| N90, 2008 | ||||||
| N90_08A13 | 2.5 | 0.364 | 19.4 | 0.349 | 5.8 | 0.618 |
| N90_08A15 | 1.0 | 0.311 | 8.1 | 0.333 | 1.5 | 0.679 |
| N90_08A16 | 1.3 | 0.354 | 11.0 | 0.462 | 3.5 | 0.545 |
| N90_08A19 | 0.372 | 0.128 | 6.2 | 0.354 | >50 | 7.8 |
| N90_08A25 | 1.3 | 0.350 | 5.6 | 0.413 | >50 | 0.752 |
| N90_08B2 | 0.703 | 0.338 | 3.9 | 0.361 | 5.7 | 0.394 |
| N90_08B4 | 2.9 | 0.236 | >50 | 0.380 | 2.5 | 2.6 |
| N90_08B6 | 0.644 | 0.293 | 5.3 | 0.434 | 3.0 | 0.255 |
| N90_08B11 | 0.143 | 0.055 | 4.7 | 0.063 | 1.6 | 0.036 |
| N90_08B16 | 0.328 | 0.196 | 7.0 | 0.259 | 2.5 | 0.472 |
| N90_08B17 | 1.7 | 0.651 | 14.5 | 0.488 | 3.0 | 1.7 |
| Breadth (n = 11) | 11 (100) | 11 (100) | 10 (91) | 11 (100) | 9 (82) | 11 (100) |
a
Breadth is shown as the number (percentage) of Env variants sensitive to the corresponding corresponding MAb or CD4-Ig or CD4-Ig.