Fig 4.

Clonotypic architecture of Gag CM9-specific CD8⁺ T cells. (A and B) TRBV and TRBJ usage, CDR3β amino acid sequences, and the relative frequencies of CD8⁺ T cell clonotypes specific for the CM9 epitope are shown for 5 macaques with strong VIA (A) and 6 macaques with weak VIA (B) at week 12 after the rAd5 boost. Colored boxes indicate public clonotypes. Public clonotypes were defined on the basis of TRB amino acid sequences that were present in more than one macaque, with reference to the present data and previously published sequences (15, 22, 23). (C) Simple linear regressions predicting the VIA of EM CD8⁺ T cells from week 12 after the rAd5 boost by the number of public clonotypes (left) and the frequency of public clonotypes (right) present in the CM9-specific CD8⁺ T cell population (n = 11). (D) Simple linear regressions predicting the VIA of CM CD8⁺ T cells from week 12 after the rAd5 boost by the frequency of public clonotypes (left) and the number of public clonotypes (right) present in the CM9-specific CD8⁺ T cell population (n = 11).