Figure 2.

Viral CyPA associates with CD147. Phytohemagglutinin-activated PBMC cultures were left untreated (lane 1) or were inoculated with equal amounts (adjusted by RT activity) of HIV-1 produced in the absence (lanes 2 and 3) or presence (lane 4) of CsA. After a 1 h incubation at 4°C, cells were transferred to 37°C for 40 min (lanes 1, 3, and 4) or left at 4°C (lane 2). Cells were lysed, and CD147 was immunoprecipitated with a goat polyclonal anti-CD147 antibody. Immunoprecipitated proteins were analyzed by Western blotting by using rabbit polyclonal anti-CyPA antibody or anti-CD147 mAb.