Fig 3.

A compensatory mutation in MA (70TI) can rescue the replication of dimerization mutants and partially restore genome dimerization. (A) PM1 T cells were infected with an equivalent of 500 RT units of WT, dimerization mutant, and MA 70TI mutant viruses. Viral replication was followed by measuring the RT activity in the culture supernatant every 2 to 4 days. 70TI, mutation in matrix at position 70; mock, mock-infected cells. (B) (Left) Native Northern blot analysis of MA 70TI mutant viruses; (right) quantification (means + standard deviations) (n = 2) of the percentages of dimeric RNA in the virions. The WT data are reported from Fig. 2A. D, dimer; M, monomer.