Table 1.
Correlation between viral replication, RNA dimerization, and packaging and the sequences and structural elements located within HIV-2 SL-1
| Virus | Viral replicationa | Genome dimerization (%)b | Packaging efficiencyc | Presence or absence of: |
|||
|---|---|---|---|---|---|---|---|
| pal | Stem B | 392-GGAG-395 | 439-GGAG-442 | ||||
| WT | ++ | 72.7 | 1.00 | + | + | + | + |
| SM4 | ++ | 60.6 | 0.51 | − | − | + | + |
| SM6 | + | 49.4 | 0.45 | + | − | + | − |
| SM9 | ++ | 63.2 | 1.10 | − | + | + | − |
| SM2 | − | 5.8 | 0.44 | − | + | − | + |
| SM5 | − | 8.9 | 0.46 | +d | − | − | + |
| SM8 | − | 5.6 | 0.38 | − | − | − | − |
a
Replication in PM1 T cells was followed by measuring the RT activity in the culture supernatant. Symbols indicate the presence (++) or absence (−) of detectable RT activity at 4 to 15 days postinfection (n = 3).
b
Percentage of dimeric genome in the virion as measured by native Northern blotting (n = 3).
c
Relative to that of the WT (set to 1) as measured by qRT-PCR (n = 3).
d
Alternative palindromic sequence in place of WT pal.