Fig 5.

The effects of zanamivir on receptor binding by chimeric proteins. A panel of RBCs with different degrees of receptor depletion was used to quantify HAD on cell monolayers expressing HPIV3-NDV (A) or NiV-NDV (B) in the absence (open squares) or presence (filled circles) of 2 mM zanamivir at 4°C (a temperature at which neuraminidase activity is negligible). The binding of each depleted RBC preparation (y axis) is expressed as a percentage of that of the control (i.e., of the amount of untreated, nondepleted RBCs bound to cells expressing the corresponding receptor binding protein). Data represent the means of results from triplicate monolayers from 3 representative experiments, with bars denoting standard deviations.