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. 2012 Apr 11;4(4):539–556. doi: 10.3390/v4040539

Figure 3.

Figure 3

DGFW inhibits viral protein and RNA synthesis. (A) An illustration of the treatment with DGFW. Cells were preadsorbed with EV71 at an m.o.i. of 5 in the presence of 0.5 mg/mL DGFW. Culture supernatants were then replaced with DMEM containing 2% FBS and DGFW. The cells were preadsorbed with virus at 4 °C between −1 to 0 h p.i. The cells were then harvested at 0, 3, 6 and 9 h p.i. for qPCR (B) and western blot (C) analysis. The quantity of viral RNA was quantified against a standard curve of a serial dilution of viral RNA. M and D indicate mock-infected cells and drug-treated cells, respectively. These are representative data from three independent experiments. The data were analyzed using two-tailed Student’s t-tests and are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001.