Fig 10.

Knockdown of H4K20me3 methyltransferase Suv420h1. (A to C) Raji cells transfected with siRNA against Suv420h1 (si-Suv420h1) or a control siRNA (si-control) were cultured for 48 h and then treated with TSA (300 nM) or vehicle DMSO (Control) for an additional 24 h. Levels of BZLF1 (A) and Suv420h1 (B) mRNA were checked by real-time RT-PCR. (C) Levels of Suv420h1 protein were examined by immunoblotting. (D to H) Epigenetic status of the Zp after Suv420h1 knockdown. (D) Raji cells treated with siRNA against Suv420h1 were subjected to ChIP assays using anti-H4K20me3 antibody, followed by DNA extraction and real-time PCR to detect the DNA fragment of the Zp. (E to H) Raji cells treated with siRNA and TSA in the same manner were subjected to ChIP assays using anti-H4K20me3, anti-H3K9Ac, anti-H3K4me3, and anti-histone H3 antibodies, followed by DNA extraction and real-time PCR to detect the DNA fragment of the Zp.