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Effect of lytic induction on cellular promoters. Raji cells were treated with TPA (20 ng/ml), A23187 (1 μM), and sodium butyrate (5 mM) (T/A/B; gray bar) or with the vehicle (Control; white bar) for 20 h. Cells then were cross-linked, and ChIP experiments were performed using anti-histone H3K27me3 (A) or anti-H4K20me3 (B) antibody, followed by DNA extraction and real-time PCR to detect DNA fragments using the primers as indicated.
