Fig 9.

Knockdown of H3K27me3 methyltransferase Ezh2. (A to C) Raji cells transfected with siRNA against Ezh2 (si-Ezh2) or a control siRNA (si-control) were cultured for 48 h and then treated with TSA (300 nM) or vehicle DMSO (Control) for an additional 24 h. Levels of BZLF1 (A) and Ezh2 (B) mRNA were checked by real-time RT-PCR. (C) Levels of Ezh2 protein were examined by immunoblotting. (D to H) Epigenetic status of the Zp after Ezh2 knockdown. (D) Raji cells treated with siRNA against Ezh2 were subjected to ChIP assays using anti-H3K27me3 antibody, followed by DNA extraction and real-time PCR to detect the DNA fragment of the Zp. (E to H) Raji cells treated with siRNA and TSA in the same manner were subjected to ChIP assays using anti-H3K27me3, anti-H3K9Ac, anti-H3K4me3, and anti-histone H3 antibodies, followed by DNA extraction and real-time PCR to detect the DNA fragment of the Zp.