Skip to main content
. 2012 May;86(9):5179–5191. doi: 10.1128/JVI.00169-12

Fig 3.

Fig 3

LANA interacts with TIP60 but does not decrease its stability or abolish TIP60 acetyltransferase activity. (A) LANA interacts with TIP60. Cell extract from HEK 293T cells transfected with expression vectors for Flag-LANA dCR and V5-TIP60 were immunoprecipitated with anti-Flag-conjugated beads. (B) LANA does not reduce TIP60 stability. Flag-TIP60 was transfected alone or with a LANA expression vector, and protein extracts were prepared from cells treated with 200 μg/ml cycloheximide for the indicated times or cycloheximide plus 10 μM MG132 for 4 h. (C and D) LANA does not ablate TIP60 acetyltransferase activity. Cells were transfected with Flag-TIP60 alone or Flag-TIP60 and LANA. Cell extracts were immunoprecipitated with anti-Flag antibody to directly precipitate Flag-TIP60 (C) or with anti-LANA antibody to obtain LANA-bound Flag-TIP60 (D), and Flag-TIP60 was assayed for acetyltransferase activity. The presence of equal amounts of TIP60 in the immunoprecipitates was determined by Western blotting (bottom).