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. 2012 May;86(9):5179–5191. doi: 10.1128/JVI.00169-12

Fig 5.

Fig 5

RPA1 and RPA2 interact with LANA and associate with the KSHV terminal repeats. (A) LANA interacts with XPA and RPA1. Cell extract from HEK 293T cells transfected with expression vectors for Flag-XPA, Flag-RPA1, and LANA were immunoprecipitated with anti-Flag-conjugated beads. (B) LANA interacts with endogenous RPA1, RPA2, and TRF1. Extracts of Flag-LANA-transfected HEK 293T cells were immunoprecipitated with anti-Flag-conjugated beads and subjected to SDS-PAGE and Western blot analysis. Coimmunoprecipitation of endogenous RPA1 (top) RPA2 (second from top) and TRF1 (second from bottom) were detected with the respective specific antibodies. Expression of LANA was detected with anti-LANA antibody (bottom). (C) RPA1, RPA2, and XPA associate with the terminal repeats. Chromatin immunoprecipitation (ChIP) assays were performed on chromatin from BC3 cells that were immunoprecipitated with anti-RPA1, anti-RPA2, anti-TRF1, or anti-XPA antibody or control IgG. The immunoprecipitated DNA was subjected to real-time PCR using primers for the KSHV terminal repeat region. The results are presented as fold enrichment relative to control IgG. TRF1 was not associated with the terminal repeats.