Fig 4.

LANAΔ(1052–1082) fails to repress RTA autoactivation in vitro. WT-LANA was able to repress RTA promoter, but LANAΔ(1052–1082) did not repress RTA induction in 293T cells (A) or in DG75 cells (B). In 293T cells, RTA repression by WT-LANA was dose dependent, while LANAΔ(1052–1082) did not repress RTA induction at three different doses (A). pRplucΔ1327 is a mutant RTA reporter with a deletion of all RBP-Jκ binding sites. Neither WT-LANA nor LANAΔ(1052–1082) repressed pRplucΔ1327 induction in 293T cells (C) or DG75 cells (D). The protein expression levels of each group were estimated by Western blotting and are shown in the lower panels. The expression of RTA, WT-LANA, or LANAΔ(1052–1082) and endogenous GAPDH were estimated. Neither WT-L ANA nor mutant LANA repressed ORF57 or ORF59 promoter autoactivation in 293T cells (E and F).