Fig 5.

LANAΔ(1052–1082) is ineffective in repressing viral lytic genes in the context of the full-length KSHV genome. qRT-PCR was used to examine viral gene expression levels at 0, 24, and 48 h after chemical induction. The relative mRNA levels were first normalized to GAPDH and then to percentages of GFP-positive cells. Four viral genes were investigated: a latent gene, vCyclin (A); an immediate-early gene, RTA (B); a delayed-early gene, PAN (C); and a late gene, TK (D). Compared to WT-LANA, LANAΔ(1052–1082) was less efficient in repressing KSHV lytic genes RTA, PAN, and TK although the patterns of repression were slightly different. Statistical analysis was conducted using a Student's t test. *, P < 0.05; **, P < 0.01.