Fig 6.

LANAΔ(1052–1082) is inefficient in maintaining the BAC36ΔLANA episome in 293T cells. WT-LANA or LANAΔ(1052–1082) was coexpressed with BAC36ΔLANA to complement the expression of LANA. At 24 and 48 h posttransfection, cells were virtualized and photographed with a fluorescence microscope (A), and the percentage of GFP-positive cells was counted using FACS (B). BAC36ΔLANA transfected with vector pCAGGS was used as a control. Both photography and FACS data indicated that there were fewer GFP-positive cells in LANAΔ(1052–1082) cells than in WT-LANA.