Fig 3.

CyPA binding to P6 peptide is conserved across all seven genotypes. (A) Amino acid sequences of the P6 and P6′ (sequence shifted one amino acid upstream of P6) peptides of the indicated genotypes (the NCBI accession numbers for the sequences selected are 1a, AAB67036.1; 1b, AJ238799.1; 2a, AB047639.1; 2b, D10988.1; 3a, ADF97231.1; 4a, CAA72338.1; 5a, AAC61696.1; 6a, CAA72801.1; 7a, ABN05226.1). Note the unique N-terminal tyrosine residue in the GT3a P6 sequence. (B) CyPA bound to P6/P6′ of different genotypes in a PPIase-dependent and CPI-sensitive manner. Ponceau S staining revealed that the differences in binding strengths of the different P6/P6′ sequences were not due to the amount of peptides spotted.