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ALPAW-containing peptides from cellular proteins interact with CyPA in a spot assay. A BLAST search of the GenBank database identified two cellular proteins, Supervillin and Nsun5, that contain a linear peptide with the ALPAW motif. The wt peptides, but not the proline-deficient mutants (sequences shown in panel A), bound to CyPA in a PPIase-dependent and DEB-025-sensitive manner (B).
