Table 1.
Sequences of CyPA-binding peptides and their proline-deficient mutantsa
| Spot name | Sequence | Reference |
|---|---|---|
| S37 | PRKGPPLPNGDTEVHGLA | 45 |
| S37P− | ARKGAALANGDTEVHGLA | This study |
| S157 | VIFDPYAPKLTSSVAEHK | 45 |
| S157P− | VIFDAYAAKLTSSVAEHK | This study |
| HIV Gag | VHAGPIAPGQMREPRGSD | 65 |
| HIV GagP− | VHAGAIAAGQMREARGSD | This study |
a
Positive-control peptides that have been described previously to bind CyPA and their derivatives devoid of proline residues were used to validate the spot-binding assay (see Fig. 1A). Alanine residues in bold indicate substitutions.